Valid in oncology, in dermo-cosmetic and cosmetic.
Pearl BioSystem is offering various assay conditions such as Apoptosis – Mitochondria as described below:
A/ Sample Testing on our Prototyping & Validated Assay:
Pearl BioSystem R&D has already developed some Assays to rapidly evaluate the added value of the pearl technology.
Human cancerous cell lines (X):
Mammary MCF-7, (ATCC® HTB-22)
Pancreatic AsPC-1 (ATCC® CRL-1682)
Pancreatic BxPC-3 (ATCC® CRL-1687)
Colon SW-620 (ATCC® CCL-227™)
Testicular NT2/D1 (ATCC® CRL-1973)
Acute monocytic leukemia cell line THP-1 (ATCC® TIB-202)
Fibroblasts BJ (ATCC® CRL-2522) = Control Cell Line
Human skin cell lines (X):
To design your assay, please choose one of the cell lines above.
Chose the number of molecules you would like to evaluate - in each case the molecule control is required and analyzed in parallel.
Receive a final quote based on number of cell lines and number of molecules to be evaluated with a time of execution.
Pearl BioSystem scientists will partner with you to develop your project requirements and assay criteria. Your target analyte may be proprietary or from a commercially available source, and you may select from several development parameters to customize your assay to your specific needs. You will receive incremental progress updates throughout the project and a final development report with your completed Discovery or Verified assay conditions. You may also choose to have Pearl BioSystem scientists perform testing on your molecules using either a prototyped or fully verified and manufactured assay.
Below an example of our accurate steps developed in our module PBS-0026 ST to qualify and sort out your molecules.
PBS-0026 - ST - Cell Line X
Sample Testing - Prototyping Estimation of EC 50 PBS Performances on cells line X
Qualified Operations of a Sample Testing Phase applied to one Molecule and your Molecule control in parallel in a validated cell line in the Pearl BioSystem catalog:
Assay Development - Preliminary estimation of assay sensitivity in PBS
Standard kit usual conditions - Includes verifying EC 50 (1 nM - 1 µM) determining on Cell Line X (X among our PBS Catalog)
- n=3 experiments not at the same day real experiment in parallel - △t = 72 hours / experiment
Each experiment n in every 2 days - Dilution in DMSO Dilutional linearity / Parallelism Determination in optimized conditions - Z' provided per experiments under PBS protocols - Dilution in DMSO Dilutional linearity / Parallelism Determination in optimized conditions - Z' provided per experiments under PBS protocols - (all raw materials included : microplate - cell lines as much as required to deliver the assay)
In this phase of Sample Testing, Christian D Muller, the Pearl BioSystem CSO will provide some clear milestones and NO/GO STEP with at the end a global report with (EC 50 - Images of the Organoids).
B/ If your cell lines are not in our developed list, our Custom Assay Development Program will develop a proprietary assay on your specific development needs.